Sulfo-NHS-SS-Biotin sodium

Research use only, not for human use.

Sulfo-NHS-SS-biotin is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent.

Sulfo-NHS-SS-biotin is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.(In Vitro):Sulfo-NHS-SS-biotin is a negatively charged reagent and does not permeate cell membranes. Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail .

Sulfo-NHS-SS-biotin is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes.Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides.Sulfo-NHS-SS-biotin has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns.Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010). Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer.

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Sulfo-NHS-SS-Biotin | Sulfo-NHS-SS-Biotin (sodium) | Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate | Sulfo-NThiol-SS-biotin

Others

Other Targets

SARS-CoV-2

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325143-98-4

606.67

C19H27N4NaO9S4

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (206.04 mM)

[H][C@]12CS[C@@H](CCCCC(=O)NCCSSCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)O[Na])[C@@]1([H])NC(=O)N2

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(-20℃)

With Ice Pack

≥ 2 years